Waterborne Pathogens Panel
Sample Collection Instructions

This panel includes culture for four (4) waterborne pathogens: 1) Legionella spp. 2) Stenotrophomonas maltophilia  3) Acinetobacter spp.  4) Pseudomonas aeruginosa
The sample volume is 500 ml and should be collected as follows:

1. Complete the requisition form provided (online form is also available at www.specialpathogenslab.com).
2. Use waterproof pen to label bottle and swab with sample location, description, and date.
3. Remove aerator if present.  Moisten the outlet by turning on the hot water – briefly. Insert swab into faucet opening rotating four (4) times against the inner surface as it moves up into the opening.
4. Turn on hot water and immediately fill the bottle (~500ml). Insert swab (taken from same location) into bottle and vigorously swirl for approximately 30 seconds and discard. Close bottle and invert to mix neutralizer.
5. Place water sample in shipping pouch with absorbent material -close pouch using zip lock seal.
6. Repeat steps 2-5 with next sample location.
    

1. Ship the same day samples are collected.
2. Sample(s) may be refrigerated (2-8° C) overnight if necessary.
3. Seal thermal pouch and place in box.
4. Place completed requisition in box (outside of thermal shipping pouch).
5. SHIP OVERNIGHT MONDAY – THURSDAY ONLY to:

Special Pathogens Laboratory
1401 Forbes Avenue, Suite 209
Pittsburgh, PA 15219

Call the lab at 412-281-5335 with any questions