Water is recognized as a major source of non-tuberculous Mycobacterium (NTM) species responsible for opportunistic infections in humans. These bacteria usually affect individuals with pre-existing conditions, specifically, lung disease, immunosuppression, transplantation and HIV infection. The most common infections are pneumonia and gastrointestinal disease caused by M. avium and M. intracellulare. Non-immunosuppressed individuals can also acquire NTM infections, most commonly, catheter sepsis, surgical and posttraumatic wound infections and cervical lymphadenitis in children.
Types of water that may be tested include: potable hot water systems where high risk patients are housed, spas and hot tubs.
Filter concentration and decontamination with 0.2 M KCL-HCL, pH 2.2. Cultivation using Middlebrook 7H10, incubation at 30° C for 6 weeks.
Turn Around Time
6 to 8 weeks
The sample volume is 200 ml and should be collected as follows:
- Complete Chain of Custody form provided in sampling kit.
- Use waterproof pen to label bottle with sample location, description, and date.
- Turn on hot water and immediately fill the bottle. Close bottle and invert to mix neutralizer.
- Place bottles in box with insulated liner.
- Ship the same day samples are collected
- Sample(s) may be refrigerated (2-8° C) overnight if necessary.
- Place bottles in box with insulated liner..
- Place completed Chain of Custody in plastic document holder and pack in box.
- Ship overnight Monday–Thursday only to: Special Pathogens Laboratory, 1401 Forbes Avenue, Suite 401, Pittsburgh, PA 15219
Questions? Call 412-281-5335